GROWTH-HORMONE (GH) STIMULATES INSULIN-LIKE GROWTH-FACTOR-I (IGF-I) AND IGF-BINDING PROTEIN (IGFBP)-2 GENE-EXPRESSION IN SPLEENS OF JUVENILE RATS

Growth and development of the spleen involves the growth hormone (GH)/ insulin-like growth factor-I (IGF-I) axis. To evaluate the molecular m echanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy an the expression of IGF-I, the IGF-I rece ptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven d ays partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA le vels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splen ic growth and development is via local (paracrine) IGF-I expression, i n addition to any effect by circulating (endocrine) IGF-I.