Wr. Cullen et al., IDENTIFICATION OF EXTRACELLULAR ARSENICAL METABOLITES IN THE GROWTH-MEDIUM OF THE MICROORGANISMS APIOTRICHUM-HUMICOLA AND SCOPULARIOPSIS-BREVICAULIS, Applied organometallic chemistry, 8(4), 1994, pp. 303-311
The separation and identification of some of the arsenic species produ
ced in cells present in the growth medium when the microorganisms Apio
trichum humicola (previously known as Candida humicola) and Scopulario
psis brevicaulis were grown in the presence of arsenicals were achieve
d by using hydride generation-gas chromatography-atomic absorption spe
ctrometry methodology (HG GC AA). Arsenite, monomethylarsonate, dimeth
ylarsinate and trimethylarsine oxide were detected following incubatio
n with arsenate. With arsenite as a substrate, the metabolites were mo
nomethylarsonate, dimethylarsinate and trimethylarsine oxide; monometh
ylarsonate afforded dimethylarsinate and trimethylarsine oxide, and di
methylarsinate afforded trimethylarsine oxide. Trimethylarsine was not
detected when the arsenic concentration was 1 ppm.