REAGENTS FOR THE PREPARATION OF 2 OLIGONUCLEOTIDES PER SYNTHESIS (TOPS(TM))

In order to increase the efficiency of use of automated DNA synthesise rs (i.e. the number of oligomers prepared per day), we have devised an d prepared novel phosphoramidite reagents that contain a linking group which, while stable under the normal synthesis conditions, is cleaved under basic conditions. When one of these linkers is introduced at th e desired position in the synthesis of an oligonucleotide, subsequent detriylation enables the synthesis of a second oligonucleotide sequenc e upon the first. During deprotection of the oligonucleotide with ammo nium hydroxide, the chain is cleaved at either side of the points of i ntroduction of the novel reagent, generating two oligonucleotides free in solution. These reagents are of particular use in applications whe re oligomers are used in pairs (such as PCR, chemical synthesis of gen es etc.) and means that an automated synthesis facility can be used mo re efficiently, without the need for operator intervention, after the working day is over.