A NEW ATP-INDEPENDENT DNA ENDONUCLEASE FROM SCHIZOSACCHAROMYCES-POMBETHAT RECOGNIZES CYCLOBUTANE PYRIMIDINE DIMERS AND 6-4-PHOTOPRODUCTS

Authors
BOWMAN KK SIDIK K SMITH CA TAYLOR JS DOETSCH PW FREYER GA
Citation
Kk. Bowman et al., A NEW ATP-INDEPENDENT DNA ENDONUCLEASE FROM SCHIZOSACCHAROMYCES-POMBETHAT RECOGNIZES CYCLOBUTANE PYRIMIDINE DIMERS AND 6-4-PHOTOPRODUCTS, Nucleic acids research, 22(15), 1994, pp. 3026-3032
Citations number
30
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
22
Issue
15
Year of publication
1994
Pages
3026 - 3032
Database
ISI
SICI code
0305-1048(1994)22:15<3026:ANADEF>2.0.ZU;2-V
Abstract
We have discovered a new DNA endonuclease in the fission yeast Schizos accharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease ( SPDE) catalyzes a single ATP-independent incision immediately 5' to th e UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recogniti on/cleavage step of a DNA excision repair pathway.