IDENTIFICATION OF RENIBACTERIUM-SALMONINARUM SURFACE-PROTEINS BY RADIOIODINATION

Authors
FREDRIKSEN A BAKKEN V
Citation
A. Fredriksen et V. Bakken, IDENTIFICATION OF RENIBACTERIUM-SALMONINARUM SURFACE-PROTEINS BY RADIOIODINATION, FEMS microbiology letters, 121(3), 1994, pp. 297-301
Citations number
16
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
121
Issue
3
Year of publication
1994
Pages
297 - 301
Database
ISI
SICI code
0378-1097(1994)121:3<297:IORSBR>2.0.ZU;2-S
Abstract
Surface exposed proteins of Renibacterium salmoninarum were identified by radiolabelling whole bacterial cells with I-125, followed by SDS-P AGE and autoradiography. The most prominent bands had molecular masses of approximately 57 kDa and 22 kDa; in addition, some less intensivel y labelled bands were detected. Polyclonal sera raised against the 22 kDa protein did not react with the 57 kDa protein. N-terminal amino ac id sequence analysis of the purified 22 kDa protein showed no similari ty with the sequence of the 57 kDa protein.