ISOLATION AND CHARACTERIZATION OF A NOVEL GENE OF THE DEAD BOX PROTEIN FAMILY WHICH IS SPECIFICALLY EXPRESSED IN GERM-CELLS OF XENOPUS-LAEVIS

We have isolated a CDNA of the DEAD protein family from a Xenopus ovar y cDNA library. Northern hybridization revealed that the mRNA correspo nding to the cDNA [XVLG1 (Xenopus vasa-like gene)] was specifically ex pressed in adult testis and ovary. The deduced amino acid sequence of XVLG1 was relatively homologous to that of Drosophila vasa. XVLG1 prot ein was found to be expressed exclusively in adult testis and ovary by immunoblotting with a monoclonal antibody against XVLG1 protein. Immu nocytological study showed that XVLG1 protein was expressed in oogonia , oocytes, spermatogonia, spermatocytes, and also in primordial germ c ells in stage 46 tadpoles. The monoclonal antibody used in this study specifically stains germ line cells and is useful both as a germ cell lineage marker and in biochemical studies of germ cells. (C) 1994 Acad emic Press, Inc.