EXPRESSION OF 3 MOUSE HOMOLOGS OF THE DROSOPHILA SEGMENT POLARITY GENE CUBITUS-INTERRUPTUS, GLI, GLI-2, AND GLI-3, IN ECTODERM-DERIVED AND MESODERM-DERIVED TISSUES SUGGESTS MULTIPLE ROLES DURING POSTIMPLANTATION DEVELOPMENT

Citation
Cc. Hui et al., EXPRESSION OF 3 MOUSE HOMOLOGS OF THE DROSOPHILA SEGMENT POLARITY GENE CUBITUS-INTERRUPTUS, GLI, GLI-2, AND GLI-3, IN ECTODERM-DERIVED AND MESODERM-DERIVED TISSUES SUGGESTS MULTIPLE ROLES DURING POSTIMPLANTATION DEVELOPMENT, Developmental biology, 162(2), 1994, pp. 402-413
Citations number
32
Categorie Soggetti
Developmental Biology",Biology
Journal title
ISSN journal
00121606
Volume
162
Issue
2
Year of publication
1994
Pages
402 - 413
Database
ISI
SICI code
0012-1606(1994)162:2<402:EO3MHO>2.0.ZU;2-5
Abstract
Three mouse genes, Gli, Gli-2, and Gli-3, which share a similar zinc f inger domain with the products of the Drosophila segment polarity gene cubitus interruptus and the Caenorhabditis elegans sex-determining ge ne tra-1 were cloned and characterized. The expression patterns during postimplantation development of the three genes were analyzed by Nort hern blot, whole-mount, and section in situ hybridizations. Expression was first detected during gastrulation in both the ectoderm and mesod erm. Later in development, their expression became more restricted in various ectoderm- and mesoderm-derived tissues and was not detectable after completion of organogenesis. Interestingly, in the developing ne ural tube, Gli showed a narrow ventral domain of expression, whereas G li-2 and Gli-3 showed a broad and dorsally restricted domain. Expressi on of these three Gli genes in various ectoderm- and mesoderm-derived tissues suggests that they play multiple roles during postimplantation development. Consistent with this hypothesis, a naturally occurring G li-3 mutation, the mouse extra-toes mutant, shows defects in both meso derm- and ectoderm-derived tissues. (C) 1994 Academic Press, Inc.