V. Malkovska et al., HUMAN T-CELLS IN HU-PBL-SCID MICE PROLIFERATE IN RESPONSE TO DAUDI LYMPHOMA AND CONFER ANTITUMOR IMMUNITY, Clinical and experimental immunology, 96(1), 1994, pp. 158-165
In vitro culture of human peripheral blood lymphocytes (PBL) with Daud
i (Burkitt lymphoma) cells results in selective proliferation of Vgamm
a9/Vdelta2 T cells with high cytotoxicity against Daudi cells. After a
doptive transfer into severe combined immunodeficient (SCID) mice, the
se cells exert specific anti-tumour activity against Daudi lymphoma. T
o test whether cytotoxic Vy9/Vdelta2 T cells are induced in SCID mice,
human PBL injected intraperitoneally were stimulated with irradiated
Daudi cells (PBL/Daudi-SCID). After 7-14 days, PBL/Daudi-SCID had a si
gnificantly higher percentage of human gammadelta T cells in their per
itoneal cavity, lymph nodes and blood than controls (PBL-SCID). DNA co
ntent analysis of T cell subsets from PBL/Daudi-SCID showed a signific
antly higher percentage of cells in S+G2+M phases of the cell cycle in
the TCR-gammadelta-1+ than in CD3+ cell population. Human cells recov
ered from PBL/Daudi-SCID showed specific cytotoxicity against Daudi ce
lls. PBL/Daudi-SCID inoculated with a lethal dose of Daudi lymphoma su
rvived significantly longer than controls. This protection was specifi
c for Daudi cells and was not mediated by murine natural killer (NK) c
ells. Thus human peripheral blood T cells grafted in SCID mice prolife
rate in response to antigen and confer specific immunity.