Ca. Ishimaru et al., IDENTIFICATION OF PARENTS SUITABLE FOR MOLECULAR MAPPING OF IMMUNITY AND RESISTANCE GENES IN SOLANUM SPECIES, American potato journal, 71(8), 1994, pp. 517-533
A survey of DNA polymorphisms and bacterial ring rot reactions in Sola
num species was conducted to identify parents that when crossed would
produce an F1 mapping population useful for identifying DNA markers ti
ghtly linked to genes for immunity or resistance to bacterial ring rot
. Solanum tuberosum, S. acaule, diploid Solanum species, doubled monop
loid lines of S. phureja, and somatic fusion hybrids between S. tubero
sum and S. brevidens were established in tissue culture from single tr
ue potato seeds or tubers and evaluated for bacterial ring rot reactio
ns in greenhouse studies with root-inoculated tissue cultured plantlet
s. Establishment of tissue culture stocks insured the availability of
homogeneous genetic material for use in bacterial ring rot assays and
genetic analyses. Presence of Clavibacter michiganense subsp. sepedoni
cum was assessed in stems by an immunofluorescence antibody assay (IFA
S). Two accessions were found to be immune to bacterial ring rot. DNA
polymorphisms were detected within and among 24 accessions evaluated w
ith 24 RFLP markers and 10 arbitrary oligonucleotide primers (RAPDs).
These results identify parents suitable for future mapping of gene(s)
for immunity or resistance to bacterial ring rot.