CONTINUOUS BEDS - THEIR APPLICABILITY FOR IMMOBILIZATION OF PROTEINS

An epoxy-activated continuous bed can be prepared for immobilization o f proteins in a simple, rapid, and cost-effective way. The concentrati on of epoxy groups on the continuous bed was as high as 600 mu mol/mL compressed bed (compression of the bed decreases the peak broadening). Human transferrin, human serum albumin and particularly urease were e mployed as model proteins. The immobilization of urease was virtually completed within 1 h in 1 M potassium phosphate, pH 7.4. The binding c apacity was 97 mg of urease/mL compressed bed. This bed is of clinical interest, since it is inexpensive to prepare and permits reproducible enzymatic determination of urea in serum and urine (the chromatograph ic step is finished within 1-2 min).