Tp. Abbott et al., ISOLATION AND PRELIMINARY CHARACTERIZATION OF LESQUERELLA-FENDLERI GUMS FROM SEED, PRESSCAKE, AND DEFATTED MEAL, Journal of agricultural and food chemistry, 42(8), 1994, pp. 1678-1685
Polysaccharide gums were separate from Lesquerella fendleri seed. Frac
turing the seed and air classification yielded, 34% by weight, fractio
ns enriched in gums. Water-swollen epidermal seed cells, after fractur
ing in a Waring Blendor and filtering, yielded a fraction that is 21%
of the seed. Crude gums from hexane-extracted meal ranged from 35 to 4
7% depending on the amount of centrifugal force used to remove seed re
sidue. Viscosity enhancement was greatest for the gums isolated from t
he fractured epidermal cells and for a gel fraction separated by a two
-step process. A typical gum has a Rha:Ara:Xyl:Man:Gal:Glc molar ratio
of 0.5:1.0:0.1:0.1:1.4:0.5 and contains 15% galacturonosyl residues,
14% protein, and 7% ash that is mainly Ca and Mg, which may serve as c
ross-linking cations for the uronic acid residues. Protein is associat
ed with the polysaccharide at a level of 10-33% in various isolates.