ISOLATION AND PRELIMINARY CHARACTERIZATION OF LESQUERELLA-FENDLERI GUMS FROM SEED, PRESSCAKE, AND DEFATTED MEAL

Citation
Tp. Abbott et al., ISOLATION AND PRELIMINARY CHARACTERIZATION OF LESQUERELLA-FENDLERI GUMS FROM SEED, PRESSCAKE, AND DEFATTED MEAL, Journal of agricultural and food chemistry, 42(8), 1994, pp. 1678-1685
Citations number
22
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
ISSN journal
00218561
Volume
42
Issue
8
Year of publication
1994
Pages
1678 - 1685
Database
ISI
SICI code
0021-8561(1994)42:8<1678:IAPCOL>2.0.ZU;2-9
Abstract
Polysaccharide gums were separate from Lesquerella fendleri seed. Frac turing the seed and air classification yielded, 34% by weight, fractio ns enriched in gums. Water-swollen epidermal seed cells, after fractur ing in a Waring Blendor and filtering, yielded a fraction that is 21% of the seed. Crude gums from hexane-extracted meal ranged from 35 to 4 7% depending on the amount of centrifugal force used to remove seed re sidue. Viscosity enhancement was greatest for the gums isolated from t he fractured epidermal cells and for a gel fraction separated by a two -step process. A typical gum has a Rha:Ara:Xyl:Man:Gal:Glc molar ratio of 0.5:1.0:0.1:0.1:1.4:0.5 and contains 15% galacturonosyl residues, 14% protein, and 7% ash that is mainly Ca and Mg, which may serve as c ross-linking cations for the uronic acid residues. Protein is associat ed with the polysaccharide at a level of 10-33% in various isolates.