S. Scarpa et al., TRANSFORMING GROWTH-FACTOR-ALPHA MODULATES REPLICATION AND DIFFERENTIATION MARKERS ON A HUMAN NEUROBLASTOMA CELL-LINE, International journal of oncology, 5(3), 1994, pp. 547-552
The effects of TGF alpha and EGF on a human neuroblastoma cell line ex
pressing EGF receptor were studied. Both growth factors were mitogenic
for neuroblastoma and, at the same time, induced a morphological diff
erentiation. TGF alpha treatment (10 ng/ml) determined an increase in
the expression of laminin and neurofilaments, both markers typical of
neuronal phenotype. Hovewer, unlike the differentiation induced by ret
inoic acid treatment, TGF alpha modulation of neuroblastoma neuronal m
arkers was not mediated by an increase in the expression of the ret on
cogene. Moreover, TGF alpha appeared to stimulate an autocrine product
ion of TGF alpha itself, as evaluated by an increase in mRNA at three
days of treatment. These data suggest a possible involvement of EGF an
d TGF alpha in a paracrine and autocrine control of neuroblastoma, pos
sibly each accomplishing similar but complementary functions.