USE OF DIRECT IN-SITU SINGLE-COPY (DISC) PCR TO PHYSICALLY MAP 5 PORCINE MICROSATELLITES

Direct in situ single-copy polymerase chain reaction (DISC-PCR) was us ed to assign and orient a linkage group to pig chromosome 1. Five micr osatellites were analyzed, and all five were successfully localized us ing this procedure. Physical data were used to orient the linkage grou p with respect to the centromere and estimate the amount of coverage o f chromosome 1. There was excellent concordance between the physical a nd linkage maps. The linear order of the microsatellites was identical , and relative distances were similar. All markers were located on the long arm of chromosome 1. Coverage was estimated at about 32%. Thus, DISC-PCR rapidly and easily assigned and ordered microsatellite marker s for which large genomic clones do not exist.