HIGH PRODUCTION OF 1,3-PROPANEDIOL FROM GLYCEROL BY CLOSTRIDIUM-BUTYRICUM VPI-3266 IN A SIMPLY CONTROLLED FED-BATCH SYSTEM

A simple fed-batch system which controls substrate feeding by measurin g the CO2 produced during the fermentation, was developped. This Fed-b atch approach allowed high production of 1,3-propanediol from glycerol by Clostridium butyricum by avoiding substrate inhibition phenomena. 65 g/l of 1,3-propanediol was produced with a productivity of 1.21 g/l .h and a yield of 0.56. The concentration of 1,3-propanediol obtained and the productivity were significantly higher than those reached in b atch culture.