A 92-KDA HUMAN IMMUNOSTIMULATORY PROTEIN

We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 mu g/kg, fully p rotected mice against a lethal inoculum of Listeria monocytogenes. Sin ce HGP.92 protected scid mice, which lack B and T lymphocytes, this in creased resistance to Listeria did not appear to be lymphocyte mediate d. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 in hibited the growth of Lewis carcinoma cells in vitro. These two activi ties appeared to depend on an oligosaccharide moiety, as they were los t after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.