MORPHINE INDUCES C-FOS AND JUNB IN STRIATUM AND NUCLEUS-ACCUMBENS VIAD-1 AND N-METHYL-D-ASPARTATE RECEPTORS

Morphine induced the c-fos and junB immediate early genes in neurons o f the medial and ventral striatum and nucleus accumbens. Induction of c-fos and junB mRNA and Fos protein was blocked by naloxone, the D-1 d opamine (DA) receptor antagonists SCH23390 and SCH39166, and the N-met hyl-D-aspartate (NMDA) glutamate receptor antagonist MK801. SCH23390 a ttenuated morphine induction of AP-1 binding in striatum, suggesting t hat c-fos and junB contribute to AP-1 binding. SCH23390 and MK801 did not block morphine induction of c-fos and junB in septum. Since the mo rphine induction of c-fos and junB in striatum and nucleus accumbens ( NA) was similar to that observed with cocaine and amphetamine, these d ata support current concepts that limbic striatum and NA are among the brain regions that mediate drug abuse. Furthermore, since DA and NMDA receptors may mediate opiate reward and opiate induction of c-fos and junB, the DA/NMDA regulation of c-fos and junB and their target genes may produce long-term changes in the striatal and NA circuits that co ntribute to opiate drug abuse.