ENANTIOMERIC SEPARATION OF AMPHETAMINE, METHAMPHETAMINE AND RING-SUBSTITUTED AMPHETAMINES BY MEANS OF A BETA-CYCLODEXTRIN-CHIRAL STATIONARY-PHASE

The enantioseparation of amphetamine, methamphetamine and various ring -substituted amphetamines by use of a chiral stationary phase carrying immobilized native beta-cyclodextrin (beta-CyD) selectors is reported . The system is evaluated for resolving the specified compounds direct ly without any derivatization and after derivatization with phenyl iso thiocyanate (PITC), naphthyl isothiocyanate (NITC) and 6-aminoquinolyl -N-hydroxysuccinimidy1 carbamate (AQC). This direct enantioseparation is compared with the features of indirect separation of diasteromeric derivatives after reaction with the optically pure Marfey's reagent em ploying a simple non-chiral alkyl-silica (RP-8) column. A selection of those methods best suited for each single amphetamine is given. Seven teen different samples of amphetamine, confiscated by the Swedisch pol ice, were analyzed with respect to their enantiomeric composition. Wit hin this set of samples synthesized by the same method no significant deviation from a racemic ratio could be observed.