COMPUTERIZED OPTIMIZATION OF GRADIENT ELUTION CONDITIONS WITH TERNARYSOLVENT MOBILE PHASES IN RPLC

In this work, an optimization procedure for gradient RPLC separation, using ternary mobile phases, is described. This procedure requires eig ht preliminary experiments in gradient elution mode to predict the ret ention surface for each solute over the whole triangular space. This i s followed by computerized calculations to determine the best ternary gradient elution profile with respect to both selectivity and analysis rime. The efficiency of this procedure from the point of view of rapi dity and of accuracy, is illustrated for the specific separation of tw elve phenyl urea herbicides.