SUBSTRATE, SUBSTRATE-ANALOG, AND INHIBITOR INTERACTIONS WITH THE FERROUS ACTIVE-SITE OF CATECHOL 2,3-DIOXYGENASE MONITORED THROUGH XAS STUDIES

Authors
BERTINI I BRIGANTI F MANGANI S NOLTING HF SCOZZAFAVA A
Citation
I. Bertini et al., SUBSTRATE, SUBSTRATE-ANALOG, AND INHIBITOR INTERACTIONS WITH THE FERROUS ACTIVE-SITE OF CATECHOL 2,3-DIOXYGENASE MONITORED THROUGH XAS STUDIES, FEBS letters, 350(2-3), 1994, pp. 207-212
Citations number
30
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
350
Issue
2-3
Year of publication
1994
Pages
207 - 212
Database
ISI
SICI code
0014-5793(1994)350:2-3<207:SSAIIW>2.0.ZU;2-O
Abstract
The interactions of catechol (substrate), 2-hydroxy-pyridine-N-oxide ( substrate analogue), and 2-bromophenol (inhibitor) with the extradiol cleaving catechol-2,3-dioxygenase from Pseudomonas putida mt-2 have be en monitored through X-ray absorption spectroscopy (XAS). The analysis of the data provides details about the mode of coordination of the su bstrate and of the inhibitors to the active site of the enzyme.