R. Kuhn et al., USE OF CAPILLARY AFFINITY ELECTROPHORESIS FOR THE DETERMINATION OF LECTIN SUGAR INTERACTIONS, Analytical biochemistry, 218(1), 1994, pp. 131-135
Capillary affinity electrophoresis was used to study the interactions
of lectins from Tetragonolobus purpureas with L-(-)-fucose 1-phosphate
. The electrophoretic mobilities of three lectin peaks were influenced
by the complexation with the negatively charged fucose derivative. A
model that allows the calculation of the complex formation constant fr
om the change in mobility at different concentrations of fucose 1-phos
phate has been derived. Calcium ions added to the buffer solution prom
oted the complex formation of the three lectins. This technique requir
ed only minute amounts of the lectins and enabled the simultaneous stu
dy of the complex formation of several proteins. (C) 1994 Academic Pre
ss, Inc.