Hungary is increasingly suffering from drought. A screening method for
identifying more water stress tolerant maize hybrids would be an inex
pensive and quick solution to eliminate yield decreases and fluctuatio
ns. For this reason a two-season field experiment with two water suppl
ies and 6 maize hybrids was carried out at Keszthely, Hungary. The tes
t methods were: a, water uptake estimated weekly by soil moisture decr
eases; b, proline accumulation as one of the responsible biochemical c
ompounds for water retention ability; c. infrathermometrical plant str
ess measurements for temporary soil-plant water status; d, grain yield
evaluation under non stress and stress conditions. Methods were evalu
ated by their selectivity and reproducibility. Each method has been pr
oved to be selective, but their trends showed substantial differences.
This fact raised the demand of complex evaluation. For instance, afte
r silking infra stress indices and grain yields showed a good correlat
ion. But yield is not the most proper basis for a comparison as yieldi
ng ability is coupled with lower drought tolerance. Tendencies are als
o influenced by genetically determined sink-source relationship Defoli
ation as a method for expressing drought tolerance was rejected becaus
e sink-source relationship disturbed the tendencies. Since the entire
probleme of drought tolerance depends on reactions to different water
supplies, the better understanding of water transport and use of maize
is necessary. For this purpose an in situ sap sampling method has bee
n developed, which is described in detailes.