RAPID DETECTION OF VIRUS GENOME FROM IMPORTED DENGUE FEVER AND DENGUEHEMORRHAGIC-FEVER PATIENTS BY DIRECT POLYMERASE CHAIN-REACTION

Authors
MORITA K MAEMOTO T HONDA S ONISHI K MURATA M TANAKA M IGARASHI A
Citation
K. Morita et al., RAPID DETECTION OF VIRUS GENOME FROM IMPORTED DENGUE FEVER AND DENGUEHEMORRHAGIC-FEVER PATIENTS BY DIRECT POLYMERASE CHAIN-REACTION, Journal of medical virology, 44(1), 1994, pp. 54-58
Citations number
28
Categorie Soggetti
Virology
Journal title
Journal of medical virology → ACNP
ISSN journal
01466615
Volume
44
Issue
1
Year of publication
1994
Pages
54 - 58
Database
ISI
SICI code
0146-6615(1994)44:1<54:RDOVGF>2.0.ZU;2-C
Abstract
Serum specimens from patients with imported dengue fever and dengue he morrhagic fever were directly subjected to reverse transcription and p olymerase chain reaction (RT-PCR) without any RNA purification. The am plified virus genome was detected within 3 hours. The results of PCR c orresponded closely to the results of virus isolation using cultured m osquito cells, suggesting that direct RT-PCR procedure greatly facilit ates rapid diagnosis of dengue infection. (C) 1994 Wiley-Liss, Inc.