MULTICENTER EVALUATION OF AUTOMATED IMMUNOTURBIDIMETRIC ASSAYS FOR MEASUREMENT OF APOLIPOPROTEIN-A-I AND APOLIPOPROTEIN-B IN SERUM AND PLASMA

Results of a multicenter evaluation of automated assays for measuremen t of apolipoproteins (apo) A-I and B with the Paramax(R) analytical sy stem are reported. Apo A-I and apo B response surface models were used to optimize concentrations of critical assay variables. overall impre cision for apo A-I controls at concentrations of 1.01-1.61 g/L was 3.7 -6.6%; overall imprecision for ape B controls at 1.00-1.61 g/L was 2.8 -6.9%. There was no interference in apo A-I measurements. Albumin conc entrations > 59 g/L resulted in a negative interference, and collectio n in sodium heparin caused a positive interference in apo B results. A po A-I and apo B assays demonstrated acceptable agreement with compara tive methods, although the Paramax apo B assay had a negative bias wit h respect to comparison methods. In 116 healthy individuals, serum ape A-I ranged from 0.97 to 2.05 g/L and serum ape B ranged from 0.51 to 1.32 g/L.