Ej. Parr et Ka. Sharkey, C-FOS-IMMUNOREACTIVITIES AND JUNB-IMMUNOREACTIVITIES IN THE ENTERIC NERVOUS-SYSTEM OF THE GUINEA-PIG ILEUM, NeuroReport, 5(13), 1994, pp. 1657-1661
WE examined the expression of c-Fos and JunB in four immunohistochemic
al subtypes of enteric neurones in the guinea-pig ileum. In whole moun
t preparations of the myenteric and submucous plexuses from isolated s
egments incubated in normal Krebs' solution, increased numbers of many
cells expressed visible c-Fos- and JunB-immunoreactivities. These inc
reases may have been associated with the process of isolation and/or i
ncubation conditions. Depolarizing stimulation by veratridine or 50 mM
K+ induced further increases of neuronal c-Fos and JunB expression wi
th no obvious subtype preference. This probably reflected a non-specif
ic activation of most enteric neurones by these stimuli and supports t
he idea that expression of c-Fos and JunB in most or all enteric neuro
nes may be a useful determinant of activation.