PREPARATION OF A SUBTRACTIVE CDNA LIBRARY ENRICHED IN CDNAS WHICH EXPRESSED AT A HIGH-LEVEL IN CULTURED SENESCENT HUMAN FIBROBLASTS

Subtracted cDNA library was prepared by subtracting [cDNA from young g rowing SV40-transformed human fibroblasts] from [cDNA from growing SV4 0-transformed fibroblasts in extended lifespan]. Isolated cDNA clones which expressed at high level in life-extended transformed cells also expressed at high level in normal senescent fibroblasts but did at low level in growing and growth-arrested young cells. Neither fibronectin nor procollagen cDNA was isolated. This cDNA library is useful for is olation of senescent-specific cDNA species which express at high level in normal senescent cells but at low level in growing and growth-arre sted young cells, avoiding growth-arrest-specific cDNAs. (C) 1994 Acad emic Press, Inc.