INSULIN AND LIPOGENESIS IN RAT ADIPOCYTES .2. EFFECT OF HIGH-LEVELS OF INSULIN AND GLUCOSE ON LIPID-SYNTHESIS IN ISOLATED RAT ADIPOCYTES

Insulin resistant states are characterized by receptor and post-recept or defects in insulin action. When the insulin resistant state progres ses, elevated levels of insulin are accompanied by increasing levels o f glucose. In a previous paper we demonstrated that treatment of isola ted adipocytes with high levels of insulin led to a decrease in insuli n binding as well as a decrease in basal and insulin-stimulated lipid synthesis. The results of the present study establish that the additio n of high concentrations of glucose in combination with a level of ins ulin, does not modify the decrease in binding of insulin to its recept or. However, the decrease in lipid synthesis previously observed in th e presence of high concentrations of insulin was completely overcome b y the presence of high glucose. (C) 1994 Academic Press, Inc.