S. Conchon et al., SYNTHETIC CDNA-ENCODING THE RAT AT(1A) RECEPTOR - A USEFUL TOOL FOR STRUCTURE-FUNCTION RELATIONSHIP ANALYSIS, Biochemical and biophysical research communications, 199(3), 1994, pp. 1347-1354
To carry out systematic structure-function studies of the rat angioten
sin II receptors by site directed mutagenesis, or production of chimer
ic receptors, we have produced a synthetic cDNA coding for the AT1a re
ceptor. The synthetic cDNA is 1101 base pairs long, and contains 49 un
ique restriction sites that are on the average 23 base pairs apart, al
lowing replacement of specific restriction fragments by synthetic coun
terparts containing the desired modified sequence. The total cDNA was
assembled in the expression vector pECE. After stable expression in Ch
inese Hamster Ovary cells, the protein encoded by this synthetic cDNA
presents a pharmacological profile and a signal transduction mechanism
indistinguishable from the wild type rat AT1a receptor. (C) 1994 Acad
emic Press, Inc.