The height fluctuations on top of the protein lysozyme adsorbed on mic
a were measured locally with an atomic force microscope operated in ta
pping mode in liquid. Height fluctuations of an apparent size of 1 nan
ometer that lasted for about 50 milliseconds were observed over lysozy
me molecules when a substrate (oligoglycoside) was present. In the pre
sence of the inhibitor chitobiose, these height fluctuations decreased
to the level without the oligoglycoside. The most straightforward int
erpretation of these results is that the height fluctuations correspon
d to the conformational changes of lysozyme during hydrolysis. It is a
lso possible, however, that the height fluctuations are, at least in p
art, the result of a different height or elasticity of the transient c
omplex of lysozyme plus the substrate.