PLANT-REGENERATION IN CALLUS-CULTURES OF FABA BEAN (VICIA-FABA L)

Callus cultures of Vicia faba L. were established using immature zygot ic embryos and shoot apices of aseptically germinated seedlings. The c alli induced on auxin-containing media (2,4-dichlorophenoxyacetic acid , 2,4-D; a-naphthaleneacetic acid, NAA) were separated from the initia l explants and subcultured in series. Regeneration of embryoids and sh oot-bud primordia was observed on auxin-containing induction media as well as after transfer of calli onto regeneration media supplemented w ith cytokinins. The shoot-bud formation capacity was stable during the long-term culture. Root formation was induced on various rooting medi a and resulted in complete flowering plants.