REGENERATION AND PEROXIDASES ACTIVITY IN CABBAGE TISSUE-CULTURE UNDERTHE INFLUENCE OF BENZOLINON AND AUXINS

There is no doubt that the plant growth regulators influence the dedif ferentiation, differentiation and growth of the plant tissues cultured in vitro. Peroxidases am known to be the reliable marker of the devel opment in tissue culture (Hoof, Gaspar, 1976; Thorpe, Gaspar, 1978; Ka y, Basile, 1984; Neuman etal.,1988; Sen et al.,1989). At the same time , the peroxidases are influenced by plant growth regulators (Gaspar et al., 1992; Neuman et al., 1988; Neuman et al., 1992). These relations can be used as a good tool for the comparison of a substance with the regulatory activity with known growth regulators. Benzolinon (3-benzy l-oxykarbonyl-methyl-benzothiazol) is a new substance which is known t o influence growth and development in vivo even in vitro but the mecha nism of the regulation of these processes is still unknown. The explan ts isolated from the cabbage seedling hypocotyls were cultured on basa l medium (Murashige, Skoog, 1962) with 1 mg.l-1 benzyladenin and 1 mg. l-1 kinetin (control) with addition of benzolinon (in Rasfim 30 DKV, a commercial preparation containing 30% of benzolinon), NAA and 2,4-D i n concentrations of 10(-4), 10(-5), 10(-6), 10(-7), 10(-8) M.l-1, resp . The comparison of the media variants and callus formation (Fig. 1), bud induction (Fig. 2), shoot and root regeneration (Figs 3, 4) sugges t that benzolinon has an auxin-like activity. The comparison of the zy mograms of anodic peroxidases (Fig. 5) and the enzyme activity (Fig. 6 ) contributes to the same conclusion. The results reveal that in the p lant tissue culture benzolinon acts as an auxin that is not so effecti ve as NAA.