A SIMPLE 2-STEP PURIFICATION OF ANTIDIABETIC COMPOUNDS FROM EUGENIA-JAMBOLANA FRUIT-PULP - PROTEOLYTIC RESISTANCE AND OTHER PROPERTIES

A simple, two-step, procedure has been developed to purify antidiabeti c compounds from Eugenia jambolana fruit-pulp. The compounds have been identified as a peptidylglycan and an oligosaccharide with molecular weights of 6.0 and 1.2 kD, respectively. The binding between the sugar s and the peptide in the peptidylglycan appears to be covalent. The am ino acid and sugar composition of the peptidylglycan have been determi ned as have the sugars of the oligosaccharide. The intrinsic color of the peptidylglycan was attributed to the compound itself and not to th e presence of pigment or metal. The peptidylglycan was resistant to de gradation by proteolytic enzymes in vitro, explaining its efficacy in oral feeding.