DETERMINATION OF COPROPORPHYRINOGEN-III OXIDASE ACTIVITY IN BACTERIA

Authors
MAMET R SCHARF R KELLER N ZIMMELS Y SCHOENFELD N
Citation
R. Mamet et al., DETERMINATION OF COPROPORPHYRINOGEN-III OXIDASE ACTIVITY IN BACTERIA, Journal of microbiological methods, 19(3), 1994, pp. 173-178
Citations number
11
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
19
Issue
3
Year of publication
1994
Pages
173 - 178
Database
ISI
SICI code
0167-7012(1994)19:3<173:DOCOAI>2.0.ZU;2-K
Abstract
A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 mug/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella p neumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg prot ein/h, respectively.