A RADIATION HYBRID MAP OF 506 STS MARKERS SPANNING HUMAN-CHROMOSOME-11

We present a high resolution radiation hybrid map-of human chromosome 11 using 506 sequence tagged sites (STSs) scored on a panel of 86 radi ation hybrids. The 506 STSs fall into 299 unique positions (average re solution of about 480 kilobases (kb)) that span pericentric markers un ambiguously assigned to the short or long arm. The map contains most g enes (125) and expressed sequence tags (26) currently assigned to chro mosome the whole chromosome. A subset of 260 STSs (143 positions) form a framework map that has a resolution of approximately 1 megabase bet ween adjacent positions and is ordered with odds of at least 1,000:1. The centromere was clearly defined with 11 and more than half of the S TSs are polymorphic microsatellite loci. These markers and the map can be used for high resolution physical and genetic mapping.