ENHANCED SENSITIVITY IN PCR DETECTION OF LISTERIA-MONOCYTOGENES IN SOFT CHEESE THROUGH USE OF AN AQUEOUS 2-PHASE SYSTEM AS A SAMPLE PREPARATION METHOD

Authors
LANTZ PG TJERNELD F BORCH E HAHNHAGERDAL B RADSTROM P
Citation
Pg. Lantz et al., ENHANCED SENSITIVITY IN PCR DETECTION OF LISTERIA-MONOCYTOGENES IN SOFT CHEESE THROUGH USE OF AN AQUEOUS 2-PHASE SYSTEM AS A SAMPLE PREPARATION METHOD, Applied and environmental microbiology, 60(9), 1994, pp. 3416-3418
Citations number
14
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
9
Year of publication
1994
Pages
3416 - 3418
Database
ISI
SICI code
0099-2240(1994)60:9<3416:ESIPDO>2.0.ZU;2-B
Abstract
A sample treatment method based on an aqueous two-phase system contain ing polyethylene glycol and dextran was developed for enhancing sensit ivity in the detection of Listeria monocytogenes in soft cheese with P CR. The results suggest that the improved detection sensitivity follow ing partitioning of the cheese homogenate in an aqueous two-phase syst em may be due to partitioning of the PCR inhibitors to the polyethylen e glycol phase.