BIOLUMINESCENT MOST-PROBABLE-NUMBER METHOD TO ENUMERATE LUX-MARKED PSEUDOMONAS-AERUGINOSA UG2LR IN SOIL

Authors
FLEMMING CA LEE H TREVORS JT
Citation
Ca. Flemming et al., BIOLUMINESCENT MOST-PROBABLE-NUMBER METHOD TO ENUMERATE LUX-MARKED PSEUDOMONAS-AERUGINOSA UG2LR IN SOIL, Applied and environmental microbiology, 60(9), 1994, pp. 3458-3461
Citations number
32
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
9
Year of publication
1994
Pages
3458 - 3461
Database
ISI
SICI code
0099-2240(1994)60:9<3458:BMMTEL>2.0.ZU;2-A
Abstract
Bioluminescence measured with a luminometer and charge-coupled device was an effective marker in most-probable number assays for luxAB-marke d Pseudomonas aeruginosa UG2Lr in soil. Most-probable-number assays wi th microtiter plate wells and luminometer tubes gave estimates for UG2 Lr that were similar to viable colony counts. Both methods detected fi ve cells per g of soil.