Am. Amaro et al., AN IMMUNOLOGICAL ASSAY FOR DETECTION AND ENUMERATION OF THERMOPHILIC BIOMINING MICROORGANISMS, Applied and environmental microbiology, 60(9), 1994, pp. 3470-3473
A specific, fast, and sensitive nonradioactive immunobinding assay for
the detection and enumeration of the moderate thermophile Thiobacillu
s caldus and the thermophilic archaeon Sulfolobus acidocaldarius was d
eveloped. It employs enhanced chemiluminescence or peroxidase-conjugat
ed immunoglobulins in a dot or slot blotting system and is very conven
ient for monitoring thermophilic bioleaching microorganisms in effluen
ts from industrial bioleaching processes.