A HETERONUCLEAR CORRELATION EXPERIMENT FOR SIMULTANEOUS DETERMINATIONOF N-15 LONGITUDINAL DECAY AND CHEMICAL-EXCHANGE RATES OF SYSTEMS IN SLOW EQUILIBRIUM
Na. Farrow et al., A HETERONUCLEAR CORRELATION EXPERIMENT FOR SIMULTANEOUS DETERMINATIONOF N-15 LONGITUDINAL DECAY AND CHEMICAL-EXCHANGE RATES OF SYSTEMS IN SLOW EQUILIBRIUM, Journal of biomolecular NMR, 4(5), 1994, pp. 727-734
A heteronuclear correlation experiment is described which permits simu
ltaneous characterization of both N-15 longitudinal decay rates and sl
ow conformational exchange rates. Data pertaining to the exchange betw
een folded and unfolded forms of an SH3 domain is used to illustrate t
he technique. Because the unfolded form of the molecule, on average, s
hows significantly higher NH exchange rates than the folded form, an a
pproach which minimizes the degree of water saturation is employed, en
abling the extraction of accurate rate constants.