A HETERONUCLEAR CORRELATION EXPERIMENT FOR SIMULTANEOUS DETERMINATIONOF N-15 LONGITUDINAL DECAY AND CHEMICAL-EXCHANGE RATES OF SYSTEMS IN SLOW EQUILIBRIUM

Citation
Na. Farrow et al., A HETERONUCLEAR CORRELATION EXPERIMENT FOR SIMULTANEOUS DETERMINATIONOF N-15 LONGITUDINAL DECAY AND CHEMICAL-EXCHANGE RATES OF SYSTEMS IN SLOW EQUILIBRIUM, Journal of biomolecular NMR, 4(5), 1994, pp. 727-734
Citations number
35
Categorie Soggetti
Biology,Spectroscopy
Journal title
ISSN journal
09252738
Volume
4
Issue
5
Year of publication
1994
Pages
727 - 734
Database
ISI
SICI code
0925-2738(1994)4:5<727:AHCEFS>2.0.ZU;2-7
Abstract
A heteronuclear correlation experiment is described which permits simu ltaneous characterization of both N-15 longitudinal decay rates and sl ow conformational exchange rates. Data pertaining to the exchange betw een folded and unfolded forms of an SH3 domain is used to illustrate t he technique. Because the unfolded form of the molecule, on average, s hows significantly higher NH exchange rates than the folded form, an a pproach which minimizes the degree of water saturation is employed, en abling the extraction of accurate rate constants.