FORMATION OF TREHALOSE AND ITS 2-DEOXY ANALOGS THROUGH CONDENSATION BY A TREHALASE FROM LOBOSPHAERA SP

A purified trehalase from Lobosphaera sp. catalyzed the condensation n ot only of D-glucose but also of 2-deoxy-D-glucose, yielding alpha,alp ha'-trehalose and 2,2'-dideoxy-alpha,alpha'-trehalose, respectively. S imultaneous incubation of D-glucose and 2-deoxy-D-glucose resulted in the formation of 2-deoxy-alpha,alpha'-trehalose in addition to the abo ve disaccharides. The reaction under various conditions showed that th e enzyme synthesized dideoxy-trehalose (yield, 16%) more than trehalos e (5%). Monodeoxy-trehalose was produced most effectively in the react ion of D-glucose and 2-deoxy-D-glucose in a weight ratio of 2:5, and a t this ratio, 6% of the total weight of two substrates was converted t o the product.