ELECTROPHORETIC KARYOTYPE AND GENE ASSIGNMENT TO CHROMOSOMES OF ASPERGILLUS-ORYZAE

Authors
KITAMOTO K KIMURA K GOMI K KUMAGAI C
Citation
K. Kitamoto et al., ELECTROPHORETIC KARYOTYPE AND GENE ASSIGNMENT TO CHROMOSOMES OF ASPERGILLUS-ORYZAE, Bioscience, biotechnology, and biochemistry, 58(8), 1994, pp. 1467-1470
Citations number
28
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
58
Issue
8
Year of publication
1994
Pages
1467 - 1470
Database
ISI
SICI code
0916-8451(1994)58:8<1467:EKAGAT>2.0.ZU;2-U
Abstract
An electrophoretic karyotype of Aspergillus oryzae was obtained by tra nsverse altering-field electrophoresis. Seven chromosomal bands were f ound, With Schizosaccharomyces pombe chromosomes as size standards, we estimated the sizes of the chromosomes to be 7.0, 5.2, 5.0, 4.5, 4.0, 3.7, and 2.8 megabase pairs (Mbp). The chromosomal DNA bands were ide ntified with use of 13 cloned genes including ribosomal DNA. The inten sity of ethidium staining and the results of Southern blotting with 10 0 random clones isolated from A. oryzae suggested that the smallest ba nd migrated as doublet and that the total genome size was approximatel y 35 Mbp.