T. Takiharamatsuura et al., EFFECTS OF PLANT-GROWTH REGULATORS AND CARBON-SOURCES ON THEANINE FORMATION IN CALLUS-CULTURES OF TEA (CAMELLIA-SINENSIS), Bioscience, biotechnology, and biochemistry, 58(8), 1994, pp. 1519-1521
Both growth and theanine accumulation in tea callus cultures were impr
oved by the combination of 4 mg/liter benzyladenine and 2 mg/liter ind
ol-3-butyric acid, but were strongly inhibited by the addition of 2,4-
dichloropbenoxyacetic acid. The optimum initial concentration of carbo
n source was 30 g/liter sucrose. Upon the addition of more than 30 g/l
iter of sucrose, the callus fresh weight was increased, but the theani
ne formation was not improved.