LOCAL INFUSION OF GLUCOSE AND INSULIN IN ISOLATED SKIN PERFUSION SITES IN ANGORA-GOATS

Six Angora goats (average BW 35.0 +/- 2 kg) were implanted bilaterally with silicon catheters in the superficial branch of the deep circumfl ex iliac artery and in the superficial branch of the deep circumflex i liac vein to determine the utilization of glucose and its insulin depe ndency in skin tissue. Intraarterial glucose infusion (Expt. 1 ) of 15 and 150 mg/h did not change glucose level in venous blood drained fro m that region. However, infusion of 750 mg/h of glucose increased veno us blood glucose level by 87.9% (66.3 vs. 124.6 mg/dl; P < 0.01). Insu lin concentration in venous blood from the treated side was significan tly lower (11.3 vs, 12.9 muU/ml; P < 0.01) than in venous blood from t he control side. Infusion of a constant dose of glucose (750 mg/h) (Ex pt. 2) in combination with a low level of insulin (100 muU/h) increase d venous glucose relative to preinfusion level ( 1 06.8 vs. 62.3 mg/dl ; P < 0.01 ). However, infusion of glucose with 1000 muU/h of insulin or 10 000 muU/h reduced (P < 0.01) the glucose level approximately to its initial value (84.2 and 69.4 mg/dl, respectively). Infusion of glu cose alone or with insulin decreased (P < 0.01) nonesterified fatty ac ids concentration in the blood from 523 muEq/l in the baseline sample to 284 muEq/l. Blood flow in the explored region varied from 164 to 22 2 ml/min. This study demonstrates that infusion of low levels of hormo nes to local skin areas can be accomplished without affecting systemic hormone and metabolite profiles.