Je. Ellis et al., POLYPEPTIDES OF HYDROGENOSOME-ENRICHED FRACTIONS FROM RUMEN CILIATE PROTOZOA AND TRICHOMONADS - IMMUNOLOGICAL STUDIES, FEMS microbiology letters, 117(2), 1994, pp. 211-216
The evolution of hydrogenosomes, energy-generating organelles of rumen
ciliate protozoa and the flagellate trichomonads has been the subject
of much speculation. Polypeptides of the hydrogenosome-enriched fract
ions from the rumen ciliates, Dasytricha ruminantium, Isostricha spp.,
Polyplastron multivesiculatum and Eudiplodinium maggii were separated
by SDS-PAGE and compared to analogous polypeptide preparations from T
ritrichomonas foetus. Immunoblotting with antisera specific to the hyd
rogenosomes of T. foetus identified common immunoreactive polypeptides
present at estimated molecular masses of 28, 35, 38, 44, 48, 58, 100
and 120 kDa. That at 120 kDa corresponds to a single subunit of the pu
rified pyruvate:ferredoxin oxidoreductase from the hydrogenosome of Tr
ichomonas vaginalis.