THE COMPLEX-FORMATION OF INFLUENZA-VIRUS ENVELOPE GLYCOPROTEINS WITH OUTER-MEMBRANE PROTEINS OF NEISSERIA-MENINGITIDIS OR BORRELIA-BURGDORFERI

The isolation of influenza virus envelope glycoproteins was achieved b y one-step procedure consisting of treatment of purified virus with zw itterionic detergent and separation of viral constituents by sucrose d ensity gradient centrifugation. Viral glycoproteins and proteins of ou ter membrane of N. meningitidis or B. burgdorferi formed complexes aft er removal of the detergent by dialysis. Complexing of viral glycoprot eins and bacterial proteins was monitored by gel chromatography on Sep harose 6B, polyacrylamide gel electrophoresis and electron microscopy. It was demonstrated by immunoblot analysis, that virus-spirochete com plexes elicited formation of antibodies in mice directed against osp A and osp B of spirochete, as well as against viral glycoproteins, resp ectively.