A SENSITIVE POLARIZED FLUOROMETER FOR CLINICAL-TESTS

Authors
LAGO P ROVATI L CORTI M
Citation
P. Lago et al., A SENSITIVE POLARIZED FLUOROMETER FOR CLINICAL-TESTS, IEEE transactions on instrumentation and measurement, 43(4), 1994, pp. 664-667
Citations number
7
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
IEEE transactions on instrumentation and measurement → ACNP
ISSN journal
00189456
Volume
43
Issue
4
Year of publication
1994
Pages
664 - 667
Database
ISI
SICI code
0018-9456(1994)43:4<664:ASPFFC>2.0.ZU;2-A
Abstract
A fully automated fluorometer which measures fluorescence depolarizati on is described. The instrument has been designed to work with standar d microtiter sample holders normally used in clinical tests. The combi nation of a hybrid optical system, built with discrete optical element s and fiber bundles, and a digital Fourier analysis of the detected si gnals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10(-3) and a minimum content of flu orescein in the sample well of 20 pmol.