NATURALLY-OCCURRING DUAL INFECTION WITH HUMAN AND BOVINE ROTAVIRUSES AS SUGGESTED BY THE RECOVERY OF G1P8 AND G1P5 ROTAVIRUSES FROM A SINGLE PATIENT

Culture adaptation of rotavirues from an infant with severe diarrhea i n Cincinnati, Ohio, yielded not only a virus with the original RNA ele ctropherotype (CJN) but also rotaviruses with other electropherotypes, the most dominant of which was called CJN-M [Ward RL, Knowlton DR, Sc hiff GM, Hoshino Y, Greenberg HB (1988) in J Virol 62: 1543-1549]. RNA -RNA hybridization and sequencing studies indicated that CJN was a typ ical G1P8 human rotavirus while CJN-M was a G1P5 strain and contained four gene segments (including segment 4) of a bovine rotavirus. Thus, the infant was apparently dually infected with human and bovine rotavi ruses.