SOME MECHANICAL-PROPERTIES OF SKINNED FIBERS OF PREGNANT HUMAN MYOMETRIUM

The properties of contractile elements and intracellular Ca2+ storage sites of pregnant human myometrium were studied by recording the mecha nical responses in skinned (saponin-treated and membrane-permeable) fi bres. Calmodulin increased the amplitude of contractions induced by Ca 2+ and the Ca2+ sensitivity for contractile elements in small myometri um strips, but PGF(2 alpha), PGE2, oxytocin, or cyclic AMP failed to p roduce similar effects. After accumulation of Ca2+ in intracellular Ca 2+ storage sites, 10 mu mol/l PGF(2 alpha), 10 mu mol/l PGE(2), 30 mmo l/l caffeine, and 20 mu mol/l InsP3 (inositol-trisphosphate) produced contractions by releasing Ca2+ from storage sites. However, 20 nmol/l oxytocin had no effects under the same conditions. The InsP3 sensitive Ca2+ store was much larger than those of PGs or caffeine. These resul ts suggest that pregnant human myometrium contracts with low Ca2+ by a calmodulin sensitive system. The data also indicate that direct appli cation of PGF(2 alpha) or PGE(2) into the cells discharges Ca2+ from C a2+ storage sites and that oxytocin extricates Ca2+ via a pathway invo lving InsP3 by activation of phosphoinositide turnover. We suggest tha t these agents induce added contractile responses due to a Ca release mechanism from store sites in addition to the influx of Ca2+ from the extracellular space.