CYTOGENETIC CHARACTERIZATION OF A PERIAMPULLARY ADENOCARCINOMA OF THEPANCREAS, ITS LIVER METASTASIS, AND A CELL-LINE ESTABLISHED FROM THE METASTASIS IN A PATIENT WITH GARDNERS-SYNDROME

A cell line was established from a liver metastasis of a periampullary pancreatic carcinoma in a patient with Gardner's syndrome. The primar y tumor, the liver metastasis, and passages 6 and 15 of the cell line were characterized cytogenetically. The only aberration common to all samples was a der(15)t(8;15); this was probably the primary chromosoma l abnormality. Loss of the short arm of chromosome 19 was also found i n all samples but was brought about by different aberrations in the pr imary tumor and the metastasis. The secondary aberrations characterist ic of clonal evolution often included further gain of 8q material but losses from 1p, 6q, and chromosomes 17 and 18, all of which have been seen before in sporadic pancreatic and colorectal carcinomas. This is the first cell line established from a tumor in a Gardner's syndrome p atient and also the first characterization of an abnormal tumor karyot ype associated with this autosomal dominant cancer syndrome.