A LOW-MOLECULAR-WEIGHT PEPTIDE FROM SNOW MOLD WITH EPITOPIC HOMOLOGY TO THE WINTER FLOUNDER ANTIFREEZE PROTEIN

Evidence for a small size protein (ca. 3500 kDa) exhibiting epitopic h omology to the Atlantic winter flounder antifreeze protein (AFP) is fo und in the snow molds Coprinus psychromorbidus, Myriosclerotinia borea lis, and Typhula incarnata. The protein shows strong cross-reactivity with antisera specific for the flounder AFP. Preliminary studies sugge st that the protein is synthesized in response to lowering the culture temperature, and that it is membrane associated and, therefore, may f unction in an analogous capacity to the fish AFP. Also, the protein is shown to have antifreeze properties as determined by nuclear magnetic resonance microimaging experiments.