Individuals of Daphnia magna Straus living together in groups were fou
nd to influence each other in a density-dependent manner. The effect a
ppeared as an inhibition in offspring production and was demonstrated
to be induced in part or completely by metabolic products released by
Daphnia itself (negative interference). Negative interference was meas
urable even when there were only two individuals in 50 ml medium (40 a
nimals l(-1)) and increased up to a density of six animals per 50 ml (
120 animals l(-1)). Also, animals living singly in Daphnia-conditioned
water showed a decrease in reproduction. For the measurements a casca
de of vessels was developed in which flow-through conditions ensured a
constant concentration of algae throughout the system.