1ST HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY OF FORMOTEROL CONCENTRATIONS IN THE LOW-PICOGRAM-PER-MILLILITER RANGE

A method for the assay of plasma concentrations of the long-acting bet a2-adrenoceptor agonist, formoterol is described. This method, in whic h high-performance liquid chromotography is used with electrochemical detection, enables, for the first time, pharmacokinetic and pharmacody namic research with this drug. After column extraction of plasma sampl es, the eluent is injected on the chromatographic system. Retention ti mes of formoterol and the internal standard, bromo-formoterol, were 5. 6 and 10 min, respectively. Our results show that this is a sensitive and reproducible method with a very low limit of detection (20 pg/ml), which creates the possibility of measuring concentrations in a range achievable in humans.